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ELK Biotechnology

ELK7082

Human CA19-5(Carbohydrate Antigen 19-5) ELISA Kit

Human CA19-5(Carbohydrate Antigen 19-5) ELISA Kit

Size
Sensitivity
Detection range

Human CA19-5(Carbohydrate Antigen 19-5) ELISA Kit

Overview

Product name: Human CA19-5(Carbohydrate Antigen 19-5) ELISA Kit
Reactivity: Human
Alternative Names: CA19-5
Assay Type: Competitive Inhibition
Sensitivity: 0.86 ng/mL
Standard: 200 ng/mL
Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Area: Tumor immunity;Hepatology;Gastroenterology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CA19-5 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA19-5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA19-5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.191 —
100.00 0.367 —
50.00 0.621 —
25.00 0.833 —
12.50 1.139 —
6.25 1.578 —
3.13 1.799 —
0.00 2.101 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CA19-5 and the recovery rates were calculated by comparing the measured value to the expected amount of CA19-5 in samples.
Matrix Recovery range Average
serum(n=5) 82-94% 88%
EDTA plasma(n=5) 97-107% 102%
Heparin plasma(n=5) 87-99% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA19-5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 90-99% 97-106% 87-98% 85-92%
EDTA plasma(n=5) 88-97% 90-99% 87-103% 82-97%
Heparin plasma(n=5) 91-99% 86-92% 91-99% 95-102%
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