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Elabscience
SKU(재고 관리 코드):E-EL-H0015
Human CA9(Carbonic Anhydrase Ⅸ) ELISA Kit
Human CA9(Carbonic Anhydrase Ⅸ) ELISA Kit
The Human CA9 (Carbonic Anhydrase Ⅸ) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the detection of Carbonic Anhydrase Ⅸ in human serum, plasma, and cell culture supernatants. This kit is intended for research purposes only and is not intended for diagnostic or therapeutic use.
The kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes a monoclonal antibody specific to human CA9. The assay is performed in a 96-well microplate coated with the capture antibody. The sample is added to the wells, and the CA9 antigen binds to the capture antibody. A biotinylated detection antibody is then added, which binds to the captured CA9 antigen. Streptavidin-HRP is added to the wells, which binds to the biotinylated detection antibody. Finally, a substrate solution is added, which reacts with the bound HRP to produce a colorimetric signal that is proportional to the amount of CA9 antigen present in the sample.
The Human CA9 ELISA Kit is highly sensitive, with a detection range of 0.156 ng/mL to 10 ng/mL. The kit has a high degree of specificity, with minimal cross-reactivity with other related proteins. The kit is easy to use, with a simple and straightforward protocol that can be completed in less than 4 hours. The kit is supplied with all necessary reagents and materials, including a detailed instruction manual.
The kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes a monoclonal antibody specific to human CA9. The assay is performed in a 96-well microplate coated with the capture antibody. The sample is added to the wells, and the CA9 antigen binds to the capture antibody. A biotinylated detection antibody is then added, which binds to the captured CA9 antigen. Streptavidin-HRP is added to the wells, which binds to the biotinylated detection antibody. Finally, a substrate solution is added, which reacts with the bound HRP to produce a colorimetric signal that is proportional to the amount of CA9 antigen present in the sample.
The Human CA9 ELISA Kit is highly sensitive, with a detection range of 0.156 ng/mL to 10 ng/mL. The kit has a high degree of specificity, with minimal cross-reactivity with other related proteins. The kit is easy to use, with a simple and straightforward protocol that can be completed in less than 4 hours. The kit is supplied with all necessary reagents and materials, including a detailed instruction manual.
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