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ELK Biotechnology

ELK9737

Human CD3(Cluster of Differentiation 3) ELISA Kit

Human CD3(Cluster of Differentiation 3) ELISA Kit

Sensitivity

Human CD3(Cluster of Differentiation 3) ELISA Kit

Overview

Product name: Human CD3(Cluster of Differentiation 3) ELISA Kit
Reactivity: Human
Alternative Names: CD3; CD3e antigen; CD3e antigen; epsilon polypeptide; TiT3 complex; CD3e molecule; epsilon; CD3-TCR complex; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex; epsilon subunit of T3; T-cell surface antigen T3; Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
Assay Type: Sandwich
Sensitivity: 0.132 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Immunology; Signal Transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 1.994 1.896
10.00 1.668 1.570
5.00 1.135 1.037
2.50 0.828 0.730
1.25 0.508 0.410
0.63 0.319 0.221
0.32 0.206 0.108
0.00 0.098 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CD3 and the recovery rates were calculated by comparing the measured value to the expected amount of CD3 in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-90% 83-96% 95-102% 92-103%
EDTA plasma(n=5) 81-92% 78-87% 91-99% 92-104%
Heparin plasma(n=5) 93-99% 86-101% 87-96% 92-105%
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