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FineTest

EH1421

Human CYP3A5(Cytochrome P450 3A5) ELISA Kit

Human CYP3A5(Cytochrome P450 3A5) ELISA Kit

size
Sensitivity
Range

CYP3A5|Cytochrome P450 3A5|Cytochrome P450-PCN3|CYPIIIA5|Cytochrome P450 HLp2

Product Name

Human CYP3A5(Cytochrome P450 3A5) ELISA Kit

Alias

CYP3A5 ELISA Kit, Cytochrome P450 3A5 ELISA Kit, Cytochrome P450-PCN3 ELISA Kit, CYPIIIA5 ELISA Kit, Cytochrome P450 HLp2 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

CYP3A5 ELISA Kit allows for the in vitro quantitative determination of CYP3A5 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Human

UniProt ID

P20815

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CYP3A5 and the recovery rates were calculated by comparing the measured value to the expected amount of CYP3A5 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

85-104

90

EDTA plasma(n=5)

90-103

99

heparin plasma(n=5)

92-103

97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYP3A5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

86-105%

85-104%

85-96%

EDTA plasma(n=5)

82-91%

88-98%

89-96%

heparin plasma(n=5)

81-89%

84-94%

82-100%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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