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ELK Biotechnology

ELK9596

Human Cys-C(CystatinC) ELISA Kit

Human Cys-C(CystatinC) ELISA Kit

Sensitivity

Human Cys-C(CystatinC) ELISA Kit

Overview

Product name: Human Cys-C(CystatinC) ELISA Kit
Reactivity: Human
Alternative Names: CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin
Assay Type: Sandwich
Sensitivity: 0.31-20 ng/mL
Standard: 20 ng/mL
Detection Range: 0.31-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Tags & Cell Markers;Cell Biology;Stem Cells;Cardiovascular
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.131 2.048
10.00 1.502 1.419
5.00 1.226 1.143
2.50 0.839 0.756
1.25 0.555 0.472
0.63 0.326 0.243
0.32 0.175 0.092
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
Matrix Recovery range Average
serum(n=5) 87-95% 91%
EDTA plasma(n=5) 93-107% 100%
Heparin plasma(n=5) 81-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-101% 86-97% 87-102% 89-101%
EDTA plasma(n=5) 89-105% 90-101% 87-93% 95-106%
Heparin plasma(n=5) 88-103% 81-98% 87-98% 95-102%
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