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RAYBIOTECH
SKU(재고 관리 코드):AAH-CYT-6
Human Cytokine Array C6
Human Cytokine Array C6
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 60 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Risk-Free Guarantee
We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
Angiogenin
|
BDNF
|
BLC
(CXCL13) |
BMP-4
|
BMP-6
|
|
Ck beta 8-1
(CCL23) |
CNTF
|
EGF
|
Eotaxin-1
(CCL11) |
Eotaxin-2
(MPIF-2/CCL24) |
|
Eotaxin-3
(CCL26) |
FGF-6
|
FGF-7
(KGF) |
Flt-3 Ligand
|
Fractalkine
(CX3CL1) |
|
GCP-2
(CXCL6) |
GDNF
|
GM-CSF
|
I-309
(TCA-3/CCL1) |
IFN-gamma
|
|
IGFBP-1
|
IGFBP-2
|
IGFBP-4
|
IGF-1
|
IL-10
|
|
IL-13
|
IL-15
|
IL-16
|
IL-1 alpha
(IL-1 F1) |
IL-1 beta
(IL-1 F2) |
|
IL-1 ra
(IL-1 F3) |
IL-2
|
IL-3
|
IL-4
|
IL-5
|
|
IL-6
|
IL-7
|
Leptin
|
Light
(TNFSF14) |
MCP-1
(CCL2) |
|
MCP-2
(CCL8) |
MCP-3
(MARC/CCL7) |
MCP-4
(CCL13) |
M-CSF
|
MDC
(CCL22) |
|
MIG
(CXCL9) |
MIP-1 delta
(CCL15) |
MIP-3 alpha
(CCL20) |
NAP-2
(PPBP/CXCL7) |
NT-3
|
|
PARC
(CCL18) |
PDGF-BB
|
RANTES
(CCL5) |
SCF
|
SDF-1 alpha
(CXCL12 alpha) |
|
TARC
(CCL17) |
TGF beta 1
|
TGF beta 3
|
TNF alpha
|
TNF beta
(TNFSF1B) |
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C6 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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