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RAYBIOTECH
SKU(재고 관리 코드):AAH-CYT-7
Human Cytokine Array C7
Human Cytokine Array C7
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 60 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Risk-Free Guarantee
We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
Adiponectin
(ACRP30) |
AgRP
|
Angiopoietin-2
|
Amphiregulin
|
Axl
|
|
bFGF
|
beta-NGF
|
Betacellulin
(BTC) |
CCL28
(MEC) |
CTACK
(CCL27) |
|
Dtk
|
EGFR
|
ENA-78
(CXCL5) |
Fas
(TNFRSF6/Apo-1) |
FGF-4
|
|
FGF-9
|
GCSF
|
GITR Ligand
(TNFSF18) |
GITR
(TNFRSF18) |
GRO alpha/beta/gamma
|
|
GRO alpha
(CXCL1) |
HCC-4
(CCL16) |
HGF
|
ICAM-1
(CD54) |
ICAM-3
(CD50) |
|
IGFBP-3
|
IGFBP-6
|
IGF-1 R
|
IL-1 R4
(ST2) |
IL-1 R1
|
|
IL-11
|
IL-12 p40
|
IL-12 p70
|
IL-17A
|
IL-2 R alpha
|
|
IL-6 R
|
IL-8
(CXCL8) |
I-TAC
(CXCL11) |
Lymphotactin
(XCL1) |
MIF
|
|
MIP-1 alpha
(CCL3) |
MIP-1 beta
(CCL4) |
MIP-3 beta
(CCL19) |
MSP alpha/beta
|
NT-4
|
|
Osteoprotegerin
(TNFRSF11B) |
Oncostatin M
|
PLGF
|
gp130
|
TNF RII
(TNFRSF1B) |
|
TNF RI
(TNFRSF1A) |
TECK
(CCL25) |
TIMP-1
|
TIMP-2
|
Thrombopoietin
(TPO) |
|
TRAIL R3
(TNFRSF10C) |
TRAIL R4
(TNFRSF10D) |
uPAR
|
VEGF-A
|
VEGF-D
|
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C7 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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