1
/
의
3
RAYBIOTECH
SKU(재고 관리 코드):AAH-CYT-8
Human Cytokine Array C8
Human Cytokine Array C8
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 54 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
Activin A
|
ALCAM
(CD166) |
CD80
(B7-1) |
BMP-5
|
BMP-7
|
|
Cardiotrophin-1
(CT-1) |
CD14
|
CXCL16
|
DR6
(TNFRSF21) |
Endoglin
(CD105) |
|
ErbB3
|
E-Selectin
|
Fas Ligand
(TNFSF6) |
ICAM-2
(CD102) |
IGF-2
|
|
IL-1 R2
|
IL-10 R beta
|
IL-13 R alpha 2
|
IL-18 BP alpha
|
IL-18 R beta
(AcPL) |
|
MMP-3
|
IL-2 R beta
(CD122) |
IL-2 R gamma
(Common gamma Chain) |
IL-21 R
|
IL-5 R alpha
|
|
IL-9
|
IP-10
(CXCL10) |
LAP/TGF beta 1
|
Leptin R
|
LIF
|
|
L-Selectin
(CD62L) |
M-CSF R
|
MMP-1
|
MMP-13
|
MMP-9
|
|
MPIF-1
(CCL23) |
NGFR
(TNFRSF16) |
PDGF-AA
|
PDGF-AB
|
PDGF R alpha
|
|
PDGF R beta
|
PECAM-1
(CD31) |
Prolactin
|
SCF R
(CD117/c-kit) |
SDF-1 beta
(CXCL12 beta) |
|
Siglec-5
(CD170) |
TGF alpha
|
TGF beta 2
|
Tie-1
|
Tie-2
|
|
TIMP-4
|
VE-Cadherin
(CDH5) |
VEGFR2
|
VEGFR3
|
|
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C8 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Share
