Human DKK3(Dickkopf Related Protein 3) ELISA Kit

The Human DKK3 (Dickkopf Related Protein 3) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative measurement of DKK3 in human serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of DKK3. The assay is performed in a 96-well microplate coated with a monoclonal antibody against DKK3. The sample is added to the wells, and the DKK3 present in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated polyclonal antibody specific for DKK3 is added to the wells.

This antibody binds to the captured DKK3, forming a sandwich complex. Streptavidin-HRP conjugate is then added to the wells, which binds to the biotinylated antibody. Finally, a substrate solution is added, and the color development is measured at 450 nm. The concentration of DKK3 in the sample is determined by comparing the optical density of the sample to the standard curve. This kit is highly specific, with no cross-reactivity with other related proteins. It is easy to use, with a short assay time of 3 hours, making it an ideal tool for research and clinical applications.