Human E2F Transcription Factor 1,E2F1 ELISA Kit

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The Human E2F Transcription Factor 1, E2F1 ELISA Kit is a highly reliable and accurate tool designed for the quantitative detection of E2F1 in human serum, plasma, and cell culture supernatant samples. This ELISA kit utilizes a solid-phase sandwich enzyme-linked immunosorbent assay principle, ensuring precise and sensitive measurement of E2F1 levels.

The E2F1 ELISA Kit includes all the necessary components for a successful assay, including pre-coated microplate wells, E2F1 standards, detection antibodies, and enzyme conjugates. The microplate wells are pre-coated with a monoclonal antibody specific to E2F1, ensuring high specificity and sensitivity. The E2F1 standards provided allow for the generation of a standard curve, enabling accurate quantification of E2F1 levels in the samples.

The assay procedure is straightforward and can be completed within a short period of time. After sample preparation and addition to the microplate wells, E2F1 in the samples binds to the immobilized antibody. Following a washing step to remove any unbound substances, a detection antibody specific to E2F1 is added, forming a sandwich complex. This complex is then detected by the addition of an enzyme conjugate, which catalyzes a colorimetric reaction. The intensity of the color developed is directly proportional to the amount of E2F1 present in the samples.

The Human E2F Transcription Factor 1, E2F1 ELISA Kit offers a wide dynamic range, allowing for the detection of E2F1 in a variety of sample types. It exhibits excellent sensitivity, with a lower limit of detection of 0.1 ng/mL. The kit also demonstrates high precision and reproducibility, ensuring reliable and consistent results.

This ELISA kit is intended for research purposes only and should not be used for diagnostic procedures. It provides a valuable tool for scientists and researchers studying the role of E2F1 in various biological processes and diseases. With its high sensitivity, accuracy, and ease of use, the Human E2F Transcription Factor 1, E2F1 ELISA Kit is an indispensable tool for quantitative analysis of E2F1 levels in human samples.