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ELK Biotechnology

ELK1008

Human EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit

Human EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit

Size
Sensitivity
Detection range

Human EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit

Overview

Product name: Human EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit
Reactivity: Human
Alternative Names: PK1; EGVEGF; PRK1, PROK1; Prokineticin 1; Black Mamba Toxin-Related Protein; Mambakine
Assay Type: Sandwich
Sensitivity: 6.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EG-VEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EG-VEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EG-VEGF in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 2.266 2.083
500.00 1.803 1.620
250.00 1.386 1.203
125.00 0.971 0.788
62.50 0.630 0.447
31.25 0.414 0.231
15.63 0.252 0.069
0.00 0.183 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant EG-VEGF and the recovery rates were calculated by comparing the measured value to the expected amount of EG-VEGF in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 79-93% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EG-VEGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 97-106% 85-98% 81-92%
EDTA plasma(n=5) 83-92% 96-102% 81-93% 79-91%
Heparin plasma(n=5) 89-101% 85-96% 95-104% 82-90%
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