Human EP2(Prostaglandin E Receptor 2) ELISA Kit

The Human EP2 (Prostaglandin E Receptor 2) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human EP2 in various biological samples. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human EP2. The sample is added to the wells, and the target protein binds to the immobilized antibody.

After washing away unbound substances, a biotinylated polyclonal antibody against human EP2 is added, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes the conversion of the substrate into a colored product, which is proportional to the amount of human EP2 present in the sample. The absorbance is measured at 450 nm using a microplate reader. This kit has a detection range of 0.156-10 ng/mL and a sensitivity of 0.094 ng/mL. It is suitable for research purposes in various fields, including immunology, oncology, and pharmacology.