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ELK Biotechnology

ELK9028

Human F-Testo(Free Testosterone) ELISA Kit

Human F-Testo(Free Testosterone) ELISA Kit

Size

Human F-Testo(Free Testosterone) ELISA Kit

Overview

Product name: Human F-Testo(Free Testosterone) ELISA Kit
Reactivity: Human
Alternative Names: F-TESTO; Free Testosterone
Assay Type: Competitive Inhibition
Sensitivity: 0.48 pg/mL
Standard: 100 pg/mL
Detection Range: 1.57-100 pg/mL
Sample Type: serum, plasma, other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F-Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-Testo in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 0.191 —
50.00 0.396 —
25.00 0.615 —
12.50 0.823 —
6.25 1.168 —
3.13 1.578 —
1.57 1.877 —
0.00 2.325 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant F-Testo and the recovery rates were calculated by comparing the measured value to the expected amount of F-Testo in samples.
Matrix Recovery range Average
serum(n=5) 80-96% 88%
EDTA plasma(n=5) 86-99% 92%
Heparin plasma(n=5) 78-92% 84%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-Testo and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-96% 93-102% 95-101% 88-96%
EDTA plasma(n=5) 85-93% 87-96% 79-91% 95-103%
Heparin plasma(n=5) 81-94% 81-95% 98-107% 96-101%
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