Human GLUT1(Glucose Transporter 1) ELISA Kit

The Human GLUT1 (Glucose Transporter 1) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative measurement of GLUT1 in human serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against GLUT1. The sample is added to the wells, and the target protein binds to the immobilized antibody.

After washing, a biotinylated polyclonal antibody against GLUT1 is added, which binds to the captured protein. The plate is then washed again, and a streptavidin-horseradish peroxidase conjugate is added, which binds to the biotinylated antibody. Finally, a chromogenic substrate is added, and the color development is measured using a microplate reader. The concentration of GLUT1 in the sample is determined by comparing the absorbance of the sample to a standard curve generated using known concentrations of recombinant GLUT1. This kit is highly specific, with no cross-reactivity with other related proteins. It is easy to use, with a short assay time of only 3 hours. This kit is ideal for research applications in the fields of diabetes, cancer, and neuroscience.