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FineTest

EH2238

Human HSD3B1(3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1) ELISA Kit

Human HSD3B1(3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1) ELISA Kit

size
Sensitivity
Range

Product Name

Human HSD3B1(3 beta-hydroxysteroid dehydrogenase/Delta 5–>4-isomerase type 1) ELISA Kit

 

Alias

HSD3B1 ELISA Kit, 3 beta-hydroxysteroid dehydrogenase ELISA Kit, Delta 5-->4-isomerase type I ELISA Kit, 3-beta-HSD I ELISA Kit, Trophoblast antigen FDO161G ELISA Kit, 3BH ELISA Kit, HSDB3A ELISA Kit

Detection Method

Competitive ELISA, Coated with Antigen

Application

HSD3B1 ELISA Kit allows for the in vitro quantitative determination of HSD3B1 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Species

Human

UniProt ID

P14060

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HSD3B1 and the recovery rates were calculated by comparing the measured value to the expected amount of HSD3B1 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

85-99

93

EDTA plasma(n=5)

89-97

94

heparin plasma(n=5)

89-105

99

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSD3B1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

89-102%

85-103%

89-105%

EDTA plasma(n=5)

82-96%

88-101%

84-99%

heparin plasma(n=5)

85-97%

81-95%

81-98%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

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