Human IL7R(Interleukin 7 Receptor) ELISA Kit

The Human IL7R (Interleukin 7 Receptor) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of human IL7R in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human IL7R.

The sample is added to the wells, and the target protein binds to the immobilized antibody. After washing away unbound substances, a biotinylated polyclonal antibody against human IL7R is added, which binds to the captured protein. Streptavidin-HRP conjugate is then added, which binds to the biotinylated antibody. Finally, a substrate solution is added, and the color development is measured at 450 nm. The concentration of human IL7R in the sample is determined by comparing the optical density of the sample to a standard curve. This kit provides a reliable and accurate tool for the detection and quantification of human IL7R, which is essential for research in immunology, oncology, and infectious diseases.