Human Inter-alpha-trypsin inhibitor heavy chain H1,ITIH1 ELISA Kit

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The Human Inter-alpha-trypsin Inhibitor Heavy Chain H1, also known as ITIH1, is a crucial component in various biological processes. This ELISA kit is specifically designed to accurately measure the concentration of ITIH1 in human samples. With its high sensitivity and precision, this kit provides reliable results for researchers and clinicians alike.

The ELISA assay utilizes a solid-phase sandwich format, where a monoclonal antibody specific to ITIH1 is pre-coated onto the microplate wells. The ITIH1 present in the sample binds to the immobilized antibody, forming an antigen-antibody complex. Following a series of washing steps to remove any unbound substances, a biotin-conjugated polyclonal antibody is added, which binds to the captured ITIH1. This is then followed by the addition of streptavidin-conjugated horseradish peroxidase (HRP), which binds to the biotin. The HRP enzyme catalyzes a colorimetric reaction upon addition of the substrate solution, resulting in a measurable signal proportional to the concentration of ITIH1 in the sample.

This ELISA kit offers a wide dynamic range, allowing for the detection of ITIH1 in various sample types, including serum, plasma, and cell culture supernatants. The assay is highly specific, with minimal cross-reactivity to other related proteins. The kit includes all necessary reagents and controls, ensuring consistent and accurate results.

With its user-friendly protocol and reliable performance, the Human Inter-alpha-trypsin Inhibitor Heavy Chain H1, ITIH1 ELISA kit is an indispensable tool for researchers and clinicians investigating the role of ITIH1 in various physiological and pathological conditions.