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ELK Biotechnology

ELK9528

Human MKRN3(makorin ring-finger protein 3) ELISA Kit

Human MKRN3(makorin ring-finger protein 3) ELISA Kit

Sensitivity

Human MKRN3(makorin ring-finger protein 3) ELISA Kit

Overview

Product name: Human MKRN3(makorin ring-finger protein 3) ELISA Kit
Reactivity: Human
Alternative Names: Probable E3 ubiquitin-protein ligase makorin-3; RING finger protein 63; Zinc finger protein 127; D15S9; RNF63; ZNF127
Assay Type: Sandwich
Sensitivity: 6.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MKRN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MKRN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MKRN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MKRN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 2.135 2.059
500.00 1.502 1.426
250.00 1.182 1.106
125.00 0.828 0.752
62.50 0.655 0.579
31.25 0.369 0.293
15.63 0.236 0.160
0.00 0.076 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MKRN3 and the recovery rates were calculated by comparing the measured value to the expected amount of MKRN3 in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 81-95% 88%
Heparin plasma(n=5) 89-103% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MKRN3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-93% 86-98% 95-102% 95-103%
EDTA plasma(n=5) 85-92% 82-96% 87-105% 89-101%
Heparin plasma(n=5) 81-98% 93-102% 78-92% 91-104%
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