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ELK Biotechnology

ELK9597

Human MR-ProADM(Mid-regional pro-adrenomedullin) ELISA Kit

Human MR-ProADM(Mid-regional pro-adrenomedullin) ELISA Kit

Sensitivity

Human MR-ProADM(Mid-regional pro-adrenomedullin) ELISA Kit

Overview

Product name: Human MR-ProADM(Mid-regional pro-adrenomedullin) ELISA Kit
Reactivity: Human
Alternative Names: MR-ProADM
Assay Type: Sandwich
Sensitivity: 9.38 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MR-ProADM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MR-ProADM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MR-ProADM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MR-ProADM in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 2.204 2.117
500.00 1.668 1.581
250.00 1.116 1.029
125.00 0.882 0.795
62.50 0.525 0.438
31.25 0.369 0.282
15.63 0.256 0.169
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MR-ProADM and the recovery rates were calculated by comparing the measured value to the expected amount of MR-ProADM in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 78-92% 85%
Heparin plasma(n=5) 78-96% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MR-ProADM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-98% 93-106% 89-97% 85-94%
EDTA plasma(n=5) 95-106% 87-101% 85-97% 95-107%
Heparin plasma(n=5) 92-103% 81-99% 87-98% 78-89%
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