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ELK Biotechnology

ELK3376

Human NKX6-1(NK6 Homeobox Protein 1) ELISA Kit

Human NKX6-1(NK6 Homeobox Protein 1) ELISA Kit

Size
Sensitivity
Detection range

Human NKX6-1(NK6 Homeobox Protein 1) ELISA Kit

Overview

Product name: Human NKX6-1(NK6 Homeobox Protein 1) ELISA Kit
Reactivity: Human
Alternative Names: NKX6A; Nkx6.1; NK6 Transcription Factor Related,Locus 1; Homeobox protein NK-6 homolog A
Assay Type: Sandwich
Sensitivity: 0.131 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX6-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX6-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX6-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX6-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.136 2.035
10.00 1.657 1.556
5.00 1.035 0.934
2.50 0.834 0.733
1.25 0.583 0.482
0.63 0.327 0.226
0.32 0.230 0.129
0.00 0.101 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NKX6-1 and the recovery rates were calculated by comparing the measured value to the expected amount of NKX6-1 in samples.
Matrix Recovery range Average
serum(n=5) 78-91% 84%
EDTA plasma(n=5) 80-96% 88%
Heparin plasma(n=5) 88-101% 94%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NKX6-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 92-105% 95-107% 82-96%
EDTA plasma(n=5) 88-104% 84-98% 95-105% 88-102%
Heparin plasma(n=5) 78-92% 83-96% 95-107% 87-102%
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