1 1

ELK Biotechnology

ELK9588

Human P-Cadherin(Cadherin, Placental) ELISA Kit

Human P-Cadherin(Cadherin, Placental) ELISA Kit

Sensitivity

Human P-Cadherin(Cadherin, Placental) ELISA Kit

Overview

Product name: Human P-Cadherin(Cadherin, Placental) ELISA Kit
Reactivity: Human
Alternative Names: CDH3; CDHP; HJMD; PCAD; Cadherin 3; type 1
Assay Type: Sandwich
Sensitivity: 37.50 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.50-4000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal Transduction;Neuroscience
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human P-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human P-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human P-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human P-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
4000.00 1.912 1.815
2000.00 1.579 1.482
1000.00 1.186 1.089
500.00 0.852 0.755
250.00 0.525 0.428
125.00 0.361 0.264
62.50 0.258 0.161
0.00 0.097 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant P-Cadherin and the recovery rates were calculated by comparing the measured value to the expected amount of P-Cadherin in samples.
Matrix Recovery range Average
serum(n=5) 80-97% 88%
EDTA plasma(n=5) 93-101% 97%
Heparin plasma(n=5) 92-106% 99%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of P-Cadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-96% 90-99% 95-106% 87-101%
EDTA plasma(n=5) 79-86% 87-103% 84-97% 88-97%
Heparin plasma(n=5) 95-106% 83-98% 79-93% 81-96%
전체 세부 정보 보기