1 1

ELK Biotechnology

ELK9496

Human Pro-ANP(Pro Atrial Natriuretic Peptide) ELISA Kit

Human Pro-ANP(Pro Atrial Natriuretic Peptide) ELISA Kit

Sensitivity

Human Pro-ANP(Pro Atrial Natriuretic Peptide) ELISA Kit

Overview

Product name: Human Pro-ANP(Pro Atrial Natriuretic Peptide) ELISA Kit
Reactivity: Human
Alternative Names: NT-proANP; Pro Atrial Natriuretic Peptide
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Pro-ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Pro-ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Pro-ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Pro-ANP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.154 2.059
5.00 1.516 1.421
2.50 1.273 1.178
1.25 0.913 0.818
0.63 0.541 0.446
0.32 0.305 0.210
0.16 0.236 0.141
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Pro-ANP and the recovery rates were calculated by comparing the measured value to the expected amount of Pro-ANP in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 92-105% 98%
Heparin plasma(n=5) 79-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pro-ANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 82-98% 93-106% 89-97%
EDTA plasma(n=5) 85-94% 95-106% 87-101% 93-106%
Heparin plasma(n=5) 82-95% 93-103% 85-94% 87-101%
전체 세부 정보 보기