FineTest
SKU(재고 관리 코드):EH2564
Human S1P(Sphingosine 1 Phosphate) ELISA Kit
Human S1P(Sphingosine 1 Phosphate) ELISA Kit
Product Name
Human S1P(Sphingosine 1 Phosphate) ELISA Kit
Alias
S1P ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
S1P ELISA Kit allows for the in vitro quantitative determination of Sphingosine 1 Phosphate|S1P concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200ng/ml
Sensitivity
< 1.875ng/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Sphingosine 1 Phosphate|S1P and the recovery rates were calculated by comparing the measured value to the expected amount of Sphingosine 1 Phosphate|S1P in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
94-104 |
100 |
|
EDTA plasma(n=5) |
87-101 |
93 |
|
heparin plasma(n=5) |
94-104 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sphingosine 1 Phosphate|S1P and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-99% |
90-105% |
87-95% |
|
EDTA plasma(n=5) |
84-95% |
85-94% |
84-94% |
|
heparin plasma(n=5) |
80-100% |
80-98% |
83-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
