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ELK Biotechnology

ELK9751

Human sAPPα (soluble amyloid precursor protein α) ELISA kit

Human sAPPα (soluble amyloid precursor protein α) ELISA kit

Sensitivity

Human sAPPα (soluble amyloid precursor protein α) ELISA kit

Overview

Product name: Human sAPPα (soluble amyloid precursor protein α) ELISA kit
Reactivity: Human
Alternative Names: sAPPα;soluble amyloid precursor protein alpha, sAPP alpha
Assay Type: Sandwich
Sensitivity: 5.15 ng/mL
Standard: 800 ng/mL
Detection Range: 12.5-800 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell culture supernates, cerebrospinal fluid (CSF)
Assay Length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sAPPα. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sAPPα. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sAPPα, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sAPPα in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
800.00 1.952 1.858
400.00 1.717 1.623
200.00 1.211 1.117
100.00 0.855 0.761
50.00 0.468 0.374
25.00 0.377 0.283
12.50 0.226 0.132
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sAPPα and the recovery rates were calculated by comparing the measured value to the expected amount of sAPPα in samples.
Matrix Recovery range Average
serum(n=5) 93-107% 100%
EDTA plasma(n=5) 81-95% 88%
Heparin plasma(n=5) 81-97% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sAPPα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 90-99% 83-97% 81-103% 85-93%
EDTA plasma(n=5) 96-103% 97-105% 89-97% 84-102%
Heparin plasma(n=5) 87-103% 94-106% 83-97% 93-102%
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