FineTest
SKU(재고 관리 코드):EH3785
Human SIRT1(Sirtuin 1) ELISA Kit
Human SIRT1(Sirtuin 1) ELISA Kit
Product Name
Human SIRT1(Sirtuin 1) ELISA Kit
Alias
SIRT1 ELISA Kit, SIR2alpha ELISA Kit, SIR2L1 ELISA Kit, SIR2-like protein 1 ELISA Kit, EC 3.5.1 ELISA Kit, S. cerevisiae ELISA Kit, homolog 1 ELISA Kit, sirtuin 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SIRT1 ELISA Kit allows for the in vitro quantitative determination of SIRT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SIRT1 and the recovery rates were calculated by comparing the measured value to the expected amount of SIRT1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
97 |
|
EDTA plasma(n=5) |
90-104 |
95 |
|
heparin plasma(n=5) |
86-104 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIRT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
85-102% |
89-104% |
|
EDTA plasma(n=5) |
82-100% |
82-94% |
88-101% |
|
heparin plasma(n=5) |
82-98% |
81-96% |
80-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
