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ELK Biotechnology

ELK9599

Human STUB1(E3 ubiquitin-protein ligase CHIP) ELISA Kit

Human STUB1(E3 ubiquitin-protein ligase CHIP) ELISA Kit

Sensitivity

Human STUB1(E3 ubiquitin-protein ligase CHIP) ELISA Kit

Overview

Product name: Human STUB1(E3 ubiquitin-protein ligase CHIP) ELISA Kit
Reactivity: Human
Alternative Names: STUB1; CHIP; HSPABP2; NY-CO-7; SCAR16; SDCCAG7; UBOX1; STIP1 homology and U-box containing protein 1
Assay Type: Sandwich
Sensitivity: 18.75 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human STUB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human STUB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human STUB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human STUB1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
2000.00 2.248 2.166
1000.00 1.638 1.556
500.00 1.157 1.075
250.00 0.828 0.746
125.00 0.516 0.434
62.50 0.321 0.239
31.25 0.216 0.134
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant STUB1 and the recovery rates were calculated by comparing the measured value to the expected amount of STUB1 in samples.
Matrix Recovery range Average
serum(n=5) 94-107% 100%
EDTA plasma(n=5) 92-107% 99%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of STUB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 93-108% 80-101% 87-96%
EDTA plasma(n=5) 88-96% 81-95% 89-97% 95-106%
Heparin plasma(n=5) 85-101% 87-102% 99-106% 78-92%
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