1 1

ELK Biotechnology

ELK9590

Human TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

Human TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

Sensitivity

Human TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

Overview

Product name: Human TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit
Reactivity: Human
Alternative Names: ACP5;TRAP5b;SPENCDI
Assay Type: Sandwich
Sensitivity: 0.47 ng/mL
Standard: 50 ng/mL
Detection Range: 0.78-50 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal Transduction;Signal Transduction;Metabolism
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50.00 2.204 2.117
25.00 1.543 1.456
12.50 1.173 1.086
6.25 0.907 0.820
3.13 0.525 0.438
1.57 0.369 0.282
0.79 0.267 0.180
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TRACP-5b and the recovery rates were calculated by comparing the measured value to the expected amount of TRACP-5b in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 96-107% 102%
Heparin plasma(n=5) 80-93% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRACP-5b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 95-103% 88-96% 81-95%
EDTA plasma(n=5) 79-96% 95-102% 82-97% 88-105%
Heparin plasma(n=5) 79-93% 87-98% 95-103% 84-98%
전체 세부 정보 보기