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ELK Biotechnology

ELK9487

Human TrkA/NTRK1(High affinity nerve growth factor receptor) ELISA Kit

Human TrkA/NTRK1(High affinity nerve growth factor receptor) ELISA Kit

Sensitivity

Human TrkA/NTRK1(High affinity nerve growth factor receptor) ELISA Kit

Overview

Product name: Human TrkA/NTRK1(High affinity nerve growth factor receptor) ELISA Kit
Reactivity: Human
Alternative Names: NTRK1; MTC; TRK; TRKA; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A;
Assay Type: Sandwich
Sensitivity: 31 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Cell Biology; Neuroscience; Signal Transduction; Developmental Biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TrkA/NTRK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TrkA/NTRK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TrkA/NTRK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TrkA/NTRK1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
5000.00 1.989 1.896
2500.00 1.607 1.514
1250.00 1.194 1.101
625.00 0.855 0.762
312.50 0.543 0.450
156.25 0.318 0.225
78.13 0.236 0.143
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TrkA/NTRK1 and the recovery rates were calculated by comparing the measured value to the expected amount of TrkA/NTRK1 in samples.
Matrix Recovery range Average
serum(n=5) 94-107% 100%
EDTA plasma(n=5) 80-92% 86%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TrkA/NTRK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-102% 89-96% 90-99% 95-106%
EDTA plasma(n=5) 87-101% 79-86% 87-103% 85-97%
Heparin plasma(n=5) 79-96% 93-105% 82-93% 95-104%
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