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SKU(재고 관리 코드):A75464
Hyaluronic Acid ELISA Kit (A75464)
Hyaluronic Acid ELISA Kit (A75464)
Name
Hyaluronic Acid ELISA Kit
Description
Hyaluronic Acid ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of Hyaluronic Acid in serum, plasma, tissue homogenates, and other biological fluids.
Assay Type
Competitive (quantitative)
Principle of Assay
Hyaluronic Acid ELISA Kit (A75464) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal Hyaluronic Acid in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Hyaluronic Acid antigen. During the incubation, Hyaluronic Acid present in the samples or standards competes with the fixed amount of immobilized Hyaluronic Acid for binding sites on the Biotinylated Anti-Hyaluronic Acid Antibody. The more Hyaluronic Acid present in a sample or standard, the less Biotinylated Anti-Hyaluronic Acid Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Hyaluronic Acid Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Hyaluronic Acid present in each sample or standard. The concentration of Hyaluronic Acid can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, tissue homogenates, and other biological fluids.
Sensitivity
0.938 ng/ml
Product Range
1.563-100 ng/ml
Assay Time
4h 30m
Reactivity
Universal
Recovery
| Sample Type | n | Range | Average |
|---|---|---|---|
| Serum | 5 | 91% - 104% | 98% |
| EDTA Plasma | 5 | 85% - 103% | 94% |
| Heparin Plasma | 5 | 89% - 105% | 95% |
Linearity
| Sample Type | n | 1:2 | 1:4 | 1:8 |
|---|---|---|---|---|
| Serum | 5 | 88-102% | 89-103% | 90-105% |
| EDTA Plasma | 5 | 82-100% | 85-95% | 85-96% |
| Heparin Plasma | 5 | 84-99% | 82-99% | 80-94% |
Precision
Intra- assay CV: < 8%, Inter-assay CV: < 10%
General Notes
Information online is representative. Always refer to the Product Manual inside the kit.
Storage
Store at +4°C. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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