Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta. AA range:141-190
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
85kD
GeneID（Human）:
1147/3551
Human Swiss-Prot No:
O15111/O14920
Cellular localization:
Cytoplasm . Nucleus . Shuttles between the cytoplasm and the nucleus.
Background:
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],
Western Blot analysis of various cells using IKKα/β Polyclonal Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IKK-alpha/beta Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HepG2 cells, using IKK-alpha/beta Antibody. The lane on the right is blocked with the synthesized peptide.