Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human IKK-gamma around the phosphorylation site of Ser31. AA range:16-65
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
48kD
GeneID（Human）:
8517
Human Swiss-Prot No:
Q9Y6K9
Cellular localization:
Cytoplasm . Nucleus . Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress. .
Background:
This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016],
Western Blot analysis of 293 cells using Phospho-IKKγ (S31) Polyclonal Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IKK-gamma (Phospho-Ser31) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with TNF-a 20ng/ml 5', using IKK-gamma (Phospho-Ser31) Antibody. The lane on the right is blocked with the phospho peptide.