Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human ITGB4 around the phosphorylation site of Tyr1510. AA range:1481-1530
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
202kD
GeneID（Human）:
3691
Human Swiss-Prot No:
P16144
Cellular localization:
Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Colocalizes with DST at the leading edge of migrating keratinocytes.
Background:
Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Western Blot analysis of AD293 using Phospho-Integrin β4 (Y1510) Polyclonal Antibody. Antibody was diluted at 1:500
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ITGB4 (Phospho-Tyr1510) Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ITGB4 (Phospho-Tyr1510) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HepG2 cells treated with Na2VO3 0.3nM 40', using ITGB4 (Phospho-Tyr1510) Antibody. The lane on the right is blocked with the phospho peptide.