Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human JunD around the phosphorylation site of Ser255. AA range:222-271
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
GeneID（Human）:
3727
Human Swiss-Prot No:
P17535
Cellular localization:
Nucleus.
Background:
The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216). [provided by RefSeq, Nov 2013],
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunD (Phospho-Ser255) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with Forskolin, using JunD (Phospho-Ser255) Antibody. The lane on the left is blocked with the phospho peptide.