Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human MEK1. AA range:252-301
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
43kD
GeneID（Human）:
5604
Human Swiss-Prot No:
Q02750
Cellular localization:
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body . Cytoplasm . Nucleus . Membrane ; Peripheral membrane protein . Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis (PubMed:14737111). Membrane localization is probably regulated by its interaction with KSR1 (PubMed:10409742). .
Background:
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008],
Western Blot analysis of 293T-UV cells using MEK-1 Polyclonal Antibody diluted at 1:500
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MEK1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from NIH/3T3 cells, treated with PMA 250ng/ml 5', using MEK1 Antibody. The lane on the right is blocked with the synthesized peptide.