Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human GRM5. AA range:21-70
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
130kD
GeneID（Human）:
2915
Human Swiss-Prot No:
P41594
Cellular localization:
Cell membrane ; Multi-pass membrane protein .
Background:
glutamate metabotropic receptor 5(GRM5) Homo sapiens This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],
Western Blot analysis of various cells using mGluR5 Polyclonal Antibody diluted at 1:1000
Western blot analysis of mouse-kidney mouse-brain lysis using mGluR5 antibody. Antibody was diluted at 1:1000
Immunofluorescence analysis of LOVO cells, using GRM5 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HT-29 cells, using GRM5 Antibody. The lane on the right is blocked with the synthesized peptide.