MLH1 Polyclonal Antibody

MLH1 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E.coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.

The MLH1 Polyclonal Antibody is a highly specific and sensitive immunological reagent designed for the detection and quantification of MLH1 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human MLH1 protein. The resulting polyclonal antibody exhibits high affinity and specificity towards MLH1 protein, making it an ideal tool for various applications in research and diagnostics.

The MLH1 Polyclonal Antibody has been extensively validated for its performance in Western blotting, immunohistochemistry, and immunofluorescence assays. It recognizes MLH1 protein from a wide range of species, including human, mouse, rat, and others. The antibody has been shown to detect endogenous MLH1 protein in various cell lines and tissues, as well as recombinant MLH1 protein expressed in different systems.

The MLH1 Polyclonal Antibody is supplied as a purified IgG fraction, which has been affinity-purified from rabbit serum using protein A chromatography. The antibody is provided in a liquid form, ready to use for various applications. It is recommended to store the antibody at -20°C in aliquots to avoid repeated freeze-thaw cycles.

In summary, the MLH1 Polyclonal Antibody is a reliable and versatile tool for the detection and quantification of MLH1 protein in various biological samples. Its high specificity and sensitivity make it an ideal choice for researchers and clinicians working in the fields of cancer biology, genetics, and diagnostics.