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SKU(재고 관리 코드):MAD295Ra21

Monoclonal Antibody to Cytochrome P450 1A1 (CYP1A1)

Monoclonal Antibody to Cytochrome P450 1A1 (CYP1A1)

UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Product No.

MAD295Ra21

Organism Species

Rattus norvegicus (Rat) .

Source

Monoclonal antibody preparation

Host

Mouse

Potency (Clone Number)

n/a

Ig Isotype

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

1mg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a mouse monoclonal antibody raised against CYP1A1. It has been selected for its ability to recognize CYP1A1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats Pubmed:25733458
The Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,3 PubMed: 25733458
Molecular Nutrition Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats Pubmed:27133590
International Journal of Chemical and Natural Science Caspase-3, Bcl-2, p53, CYP1A1 and COX -2 as a potential target in chemoprevention of Benzo (a) pyrene-induced lung carcinogenesis in mice: Role of thymoquinone portal:uploads
International Journal of Pharma Sciences Chemopreventive Role of Curcumin in benzo(A)pyrene induced Lung Carcinogenesis in mice via-modulation of Bcl-2, p53, Caspase-3, Cyp1A1, COX-2 and antioxidant defense system in Lung tissues portal:uploads
Molecular Nutrition & Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. pubmed:27133590
Biochemistry (Mosc) Cytochrome P450 1A1 (CYP1A1) Catalyzes Lipid Peroxidation of Oleic Acid-Induced HepG2 Cells Pubmed:29738693
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD295Ra01 Recombinant Cytochrome P450 1A1 (CYP1A1) Positive Control; Immunogen; SDS-PAGE; WB.
APD295Ra01 Active Cytochrome P450 1A1 (CYP1A1) Cell culture; Activity Assays.
PAD295Ra01 Polyclonal Antibody to Cytochrome P450 1A1 (CYP1A1) WB; IHC; ICC; IP.
MAD295Ra21 Monoclonal Antibody to Cytochrome P450 1A1 (CYP1A1) WB; IHC; ICC; IP.
SED295Ra ELISA Kit for Cytochrome P450 1A1 (CYP1A1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD295Ra Magnetic Luminex Assay Kit for Cytochrome P450 1A1 (CYP1A1) ,etc. Magnetic Luminex Assay for Antigen Detection.
KSD295Ra01 ELISA Kit DIY Materials for Cytochrome P450 1A1 (CYP1A1) Main materials for "Do It (ELISA Kit) Yourself".
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