Cloud-clone
SKU(재고 관리 코드):MAL154Ra21
Monoclonal Antibody to Fibroblast Growth Factor 15 (FGF15)
Monoclonal Antibody to Fibroblast Growth Factor 15 (FGF15)
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAL154Ra21
Organism Species
Rattus norvegicus (Rat).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
D3
Ig Isotype
IgG2b Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPL154Ra01-Recombinant Fibroblast Growth Factor 15 (FGF15)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against FGF15. It has been selected for its ability to recognize FGF15 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Endocrinology. | Naringenin Prevents Obesity, Hepatic Steatosis, and Glucose Intolerance in Male Mice Independent of Fibroblast Growth Factor 21 PubMed: 25774553 |
| Inflammatory Bowel Diseases | Alterations in Enterohepatic Fgf15 Signaling and Changes in Bile Acid Composition Depend on Localization of Murine Intestinal Inflammation pubmed:27580383 |
| J Lipid Res. | Commensal bacteria at the crossroad between cholesterol homeostasis and chronic inflammation in atherosclerosis. pubmed:28130274 |
| BMC Endocrine Disorders | The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance 10.1186/s12902-017-0211-5 |
| Journal of Physiology and Biochemistry | The ileal FGF15/19 to hepatic FGFR4 axis regulates liver regeneration after partial hepatectomy in mice Pubmed:29468415 |
| Developmental Cell | FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis Pubmed: 30745141 |
| Endocrinology | Interactions between the gravitostat and the fibroblast growth factor system for the regulation of body weight |
| Food & Function | Lactoferrin promotes bile acid metabolism and reduces hepatic cholesterol deposition by inhibiting the farnesoid X receptor (FXR)-mediated enterohepatic axis Pubmed: 31626262 |
| Journal of Translational Medicine | Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation Pubmed: 33036625 |
| Chin Med | Si-Wu-Tang ameliorates fibrotic liver injury via modulating intestinal microbiota and bile acid homeostasis 34736501 |
| Int J Biol Sci | Dynamics of the gut-liver axis in rats with varying fibrosis severity Pubmed:35637968 |
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