Monoclonal Antibody to Protein Tyrosine Phosphatase Receptor Type C (CD45)

Western blotting: 0.5-5µg/mL;1:200-2000
Immunohistochemistry: 5-20µg/mL;1:20-200
Immunocytochemistry: 5-20µg/mL;1:20-200
Optimal working dilutions must be determined by end user.

Product No.

MAB030Hu22

Organism Species

Homo sapiens (Human).

Source

Monoclonal antibody preparation

Host

Mouse

Potency (Clone Number)

C8

Ig Isotype

IgG1 Kappa

Purification

Protein A + Protein G affinity chromatography

Label

None

Immunogen

RPB030Hu01-Recombinant Protein Tyrosine Phosphatase Receptor Type C (CD45)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

1mg/ml

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a mouse monoclonal antibody raised against CD45. It has been selected for its ability to recognize CD45 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;1:200-2000;
Immunohistochemistry: 5-20µg/mL;1:20-200;
Immunocytochemistry: 5-20µg/mL;1:20-200;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.