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ELK Biotechnology

ELK9760

Mouse ACA-IgM(Anti-Cardiolipin Antibody IgM) ELISA Kit

Mouse ACA-IgM(Anti-Cardiolipin Antibody IgM) ELISA Kit

Sensitivity

Mouse ACA-IgM(Anti-Cardiolipin Antibody IgM) ELISA Kit

Overview

Product name: Mouse ACA-IgM(Anti-Cardiolipin Antibody IgM) ELISA Kit
Reactivity: Mouse
Alternative Names: ACA-IgM; Anti-Cardiolipin Antibody IgM
Assay Type: Sandwich
Sensitivity: 0.082 ng/mL
Standard: 20 ng/mL
Detection Range: 0.31-20 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACA-IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACA-IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACA-IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACA-IgM in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.135 2.041
10.00 1.668 1.574
5.00 1.211 1.117
2.50 0.796 0.702
1.25 0.614 0.520
0.63 0.318 0.224
0.32 0.235 0.141
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ACA-IgM and the recovery rates were calculated by comparing the measured value to the expected amount of ACA-IgM in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 84-98% 91%
Heparin plasma(n=5) 90-105% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACA-IgM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-106% 82-95% 85-96% 84-98%
EDTA plasma(n=5) 82-97% 88-105% 91-99% 81-98%
Heparin plasma(n=5) 85-97% 93-101% 85-98% 90-101%
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