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ELK Biotechnology

ELK9620

Mouse Ang1-7(Angiotensin1-7) ELISA Kit

Mouse Ang1-7(Angiotensin1-7) ELISA Kit

Sensitivity

Mouse Ang1-7(Angiotensin1-7) ELISA Kit

Overview

Product name: Mouse Ang1-7(Angiotensin1-7) ELISA Kit
Reactivity: Mouse
Alternative Names: Angiotensin 1-7
Assay Type: Sandwich
Sensitivity: 18.75 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular;Signal Transduction;Metabolism
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Ang1-7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Ang1-7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Ang1-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Ang1-7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
2000.00 1.972 1.875
1000.00 1.663 1.566
500.00 1.108 1.011
250.00 0.876 0.779
125.00 0.576 0.479
62.50 0.385 0.288
31.25 0.226 0.129
0.00 0.097 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Ang1-7 and the recovery rates were calculated by comparing the measured value to the expected amount of Ang1-7 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 84-96% 90%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ang1-7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 85-94% 84-98% 86-93%
EDTA plasma(n=5) 88-92% 86-97% 90-101% 92-102%
Heparin plasma(n=5) 85-97% 95-107% 85-94% 82-96%
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