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ELK Biotechnology

ELK9717

Mouse CA19-9(CarbohydMousee Antigen 19-9) ELISA Kit

Mouse CA19-9(CarbohydMousee Antigen 19-9) ELISA Kit

Sensitivity

Mouse CA19-9(CarbohydMousee Antigen 19-9) ELISA Kit

Overview

Product name: Mouse CA19-9(CarbohydMousee Antigen 19-9) ELISA Kit
Reactivity: Mouse
Alternative Names: sialylated Lewis(a)Antigen; Cancer Antigen 19-9
Assay Type: Sandwich
Sensitivity: 0.28 U/mL
Standard: 50 U/mL
Detection Range: 0.79-50 U/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Tumor immunity;Hepatology;Gastroenterology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50.00 2.096 2.003
25.00 1.527 1.434
12.50 1.225 1.132
6.25 0.861 0.768
3.13 0.577 0.484
1.57 0.318 0.225
0.79 0.236 0.143
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CA19-9 and the recovery rates were calculated by comparing the measured value to the expected amount of CA19-9 in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 84-93% 89%
Heparin plasma(n=5) 97-107% 102%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA19-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 90-96% 87-1028% 85-97%
EDTA plasma(n=5) 96-105% 87-96% 93-108% 92-105%
Heparin plasma(n=5) 91-99% 81-93% 78-91% 87-98%
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