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ELK Biotechnology

ELK9415

Mouse CDNF(Cerebral Dopamine Neurotrophic Factor) ELISA Kit

Mouse CDNF(Cerebral Dopamine Neurotrophic Factor) ELISA Kit

Sensitivity

Mouse CDNF(Cerebral Dopamine Neurotrophic Factor) ELISA Kit

Overview

Product name: Mouse CDNF(Cerebral Dopamine Neurotrophic Factor) ELISA Kit
Reactivity: Mouse
Alternative Names: ARMETL1; ARMET-like protein 1; Conserved Dopamine Neurotrophic Factor; Arginine-Rich,Mutated In Early Stage Tumors-Like 1
Assay Type: Sandwich
Sensitivity: 15.3 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CDNF in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
2000.00 2.201 2.107
1000.00 1.612 1.518
500.00 1.052 0.958
250.00 0.864 0.770
125.00 0.563 0.469
62.50 0.318 0.224
31.25 0.235 0.141
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CDNF and the recovery rates were calculated by comparing the measured value to the expected amount of CDNF in samples.
Matrix Recovery range Average
serum(n=5) 92-106% 99%
EDTA plasma(n=5) 81-97% 89%
Heparin plasma(n=5) 78-96% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-92% 89-98% 85-94% 87-98%
EDTA plasma(n=5) 85-92% 87-101% 93-106% 91-99%
Heparin plasma(n=5) 81-98% 78-90% 91-99% 97-103%
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