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ELK Biotechnology

ELK9621

Mouse Cys-C(CystatinC) ELISA Kit

Mouse Cys-C(CystatinC) ELISA Kit

Sensitivity

Mouse Cys-C(CystatinC) ELISA Kit

Overview

Product name: Mouse Cys-C(CystatinC) ELISA Kit
Reactivity: Mouse
Alternative Names: CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin
Assay Type: Sandwich
Sensitivity: 9.38 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Neuroscience;Stem Cells
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 2.131 2.044
500.00 1.638 1.551
250.00 1.196 1.109
125.00 0.828 0.741
62.50 0.563 0.476
31.25 0.389 0.302
15.63 0.213 0.126
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 84-106% 100%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-98% 95-104% 93-102% 83-97%
EDTA plasma(n=5) 82-96% 86-98% 93-101% 87-96%
Heparin plasma(n=5) 92-105% 85-102% 80-101% 79-96%
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