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ELK Biotechnology

ELK9609

Mouse D-LDH(D-LactateDehydrogenase) ELISA Kit

Mouse D-LDH(D-LactateDehydrogenase) ELISA Kit

Sensitivity

Mouse D-LDH(D-LactateDehydrogenase) ELISA Kit

Overview

Product name: Mouse D-LDH(D-LactateDehydrogenase) ELISA Kit
Reactivity: Mouse
Alternative Names: DLD; LDHD; Probable D-lactate dehydrogenase;mitochondrial;
Assay Type: Sandwich
Sensitivity: 2.81 mIU/mL
Standard: 300 mIU/mL
Detection Range: 4.69-300 mIU/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal Transduction;Cancer;Kits/ Lysates/ Other;Metabolism
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse D-LDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse D-LDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse D-LDH in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
300.00 2.154 2.073
150.00 1.612 1.531
75.00 1.115 1.034
37.50 0.933 0.852
18.75 0.547 0.466
9.38 0.323 0.242
4.69 0.175 0.094
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant D-LDH and the recovery rates were calculated by comparing the measured value to the expected amount of D-LDH in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of D-LDH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 82-94% 86-96% 81-97%
EDTA plasma(n=5) 92-105% 83-92% 86-93% 88-92%
Heparin plasma(n=5) 86-97% 90-101% 82-96% 93-101%
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