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ELK Biotechnology

ELK9604

Mouse E-Cad(E-Cadherin) ELISA Kit

Mouse E-Cad(E-Cadherin) ELISA Kit

Sensitivity

Mouse E-Cad(E-Cadherin) ELISA Kit

Overview

Product name: Mouse E-Cad(E-Cadherin) ELISA Kit
Reactivity: Mouse
Alternative Names: CDH1; Arc-1; CD324; CDHE; LCAM; UVO; CAM 120/80; Epithelial Cadherin; Uvomorulin
Assay Type: Sandwich
Sensitivity: 37.50 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.50-4000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal Transduction;Cancer;Developmental Biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
4000.00 2.179 2.091
2000.00 1.607 1.519
1000.00 1.140 1.052
500.00 0.852 0.764
250.00 0.527 0.439
125.00 0.305 0.217
62.50 0.252 0.164
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant E-Cad and the recovery rates were calculated by comparing the measured value to the expected amount of E-Cad in samples.
Matrix Recovery range Average
serum(n=5) 84-96% 90%
EDTA plasma(n=5) 96-107% 101%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E-Cad and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-98% 93-102% 89-97% 85-94%
EDTA plasma(n=5) 95-102% 87-103% 89-102% 87-102%
Heparin plasma(n=5) 85-94% 87-101% 87-101% 82-93%
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