Mouse FIL1d(Interleukin 1 Delta) ELISA Kit

The Mouse FIL1d (Interleukin 1 Delta) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of mouse FIL1d in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes a monoclonal antibody specific to mouse FIL1d coated onto a microplate. The sample is added to the microplate, and any FIL1d present in the sample binds to the immobilized antibody.

After washing away any unbound substances, a biotinylated monoclonal antibody specific to mouse FIL1d is added to the microplate, which binds to the FIL1d captured by the first antibody. Following another washing step, streptavidin-conjugated horseradish peroxidase is added to the microplate, which binds to the biotinylated antibody. Finally, a substrate solution is added to the microplate, which reacts with the bound enzyme to produce a colorimetric signal that is proportional to the amount of FIL1d present in the sample. This kit is highly specific, with no cross-reactivity observed with other cytokines or growth factors. It is easy to use, with a short assay time of only 3 hours, making it an ideal tool for researchers studying the role of FIL1d in various biological processes.