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ELK Biotechnology

ELK9612

Mouse IL-27(Interleukin 27) ELISA Kit

Mouse IL-27(Interleukin 27) ELISA Kit

Sensitivity

Mouse IL-27(Interleukin 27) ELISA Kit

Overview

Product name: Mouse IL-27(Interleukin 27) ELISA Kit
Reactivity: Mouse
Alternative Names: IL-27; IL-27A; IL27p28; IL30; MGC71873; p28;
Assay Type: Sandwich
Sensitivity: 18.75 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Immunology;Cancer
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-27 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
2000.00 2.135 2.054
1000.00 1.663 1.582
500.00 1.226 1.145
250.00 0.852 0.771
125.00 0.539 0.458
62.50 0.313 0.232
31.25 0.235 0.154
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL-27 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-27 in samples.
Matrix Recovery range Average
serum(n=5) 82-94% 88%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 84-93% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-93% 94-106% 83-97% 93-102%
EDTA plasma(n=5) 89-97% 85-97% 87-96% 88-97%
Heparin plasma(n=5) 91-97% 81-93% 89-103% 82-91%
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