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ELK Biotechnology

ELK2401

Mouse NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

Mouse NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

Size
Sensitivity
Detection range

Mouse NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

Overview

Product name: Mouse NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit
Reactivity: Mouse
Alternative Names: NT-Pro-ANP; N-ANP
Assay Type: Sandwich
Sensitivity: 29 pg/mL
Standard: 5000 pg/mL
Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
5000.00 1.983 1.894
2500.00 1.638 1.549
1250.00 1.088 0.999
625.00 0.728 0.639
312.50 0.589 0.500
156.25 0.239 0.150
78.13 0.168 0.079
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NT-ProANP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProANP in samples.
Matrix Recovery range Average
serum(n=5) 93-107% 100%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 85-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 82-96% 87-105% 90-99%
EDTA plasma(n=5) 88-102% 86-92% 81-93% 79-87%
Heparin plasma(n=5) 79-94% 87-96% 85-98% 95-104%
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