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ELK Biotechnology

ELK9618

Mouse pMAPT/pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit

Mouse pMAPT/pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit

Sensitivity

Mouse pMAPT/pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit

Overview

Product name: Mouse pMAPT/pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit
Reactivity: Mouse
Alternative Names: pMAPT/pTAU
Assay Type: Sandwich
Sensitivity: 9.38 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cell Biology;Cancer;Metabolism
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse pMAPT/pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse pMAPT/pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse pMAPT/pTAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse pMAPT/pTAU in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 1.929 1.843
500.00 1.587 1.501
250.00 1.116 1.030
125.00 0.839 0.753
62.50 0.614 0.528
31.25 0.387 0.301
15.63 0.215 0.129
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant pMAPT/pTAU and the recovery rates were calculated by comparing the measured value to the expected amount of pMAPT/pTAU in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 88-102% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pMAPT/pTAU and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-96% 86-93% 82-95% 80-94%
EDTA plasma(n=5) 90-99% 87-103% 83-96% 91-99%
Heparin plasma(n=5) 89-97% 95-105% 87-102% 92-102%
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